PANC1细胞ZYG11A基因过表达细胞株 | 胰腺癌ZYG11A泛素连接酶功能研究应用方案

ZYG11A基因过表达细胞系产品介绍

ZYG11A（Zyg-11 Homolog A）是Cullin 2-RING E3泛素连接酶复合体（CRL2）的底物识别亚基，与ZYG11B同属ZYG-11家族。ZYG11A通过识别底物蛋白N端甘氨酸残基（Gly/N-degron），介导泛素-蛋白酶体途径的靶向降解，在细胞周期调控、有丝分裂滑移及蛋白稳态维持中发挥关键作用。研究表明，ZYG11A在多种肿瘤中的表达水平与细胞增殖、周期进程和侵袭转移密切相关。在肺癌中，ZYG11A高表达与不良预后相关，通过调控CCNE1表达促进肿瘤细胞增殖；在卵巢癌中，ZYG11A低表达与高分级肿瘤相关，提示其可能具有抑癌功能；在子宫内膜癌中，ZYG11A受IGF1/p53信号通路调控，参与肿瘤发生发展。此外，ZYG11A基因突变可导致胰岛β细胞周期停滞，与家族性糖尿病发病相关。Western blot验证显示，ZYG11A过表达PANC1细胞系中ZYG11A蛋白水平显著升高，可用于深入研究ZYG11A在胰腺癌中的生物学功能及其调控下游靶蛋白降解的分子机制。

产品应用前景：ZYG11A过表达

利用ZYG11A过表达PANC1细胞系，可系统开展以下研究方向：1. /CRL2ZYG11A介导的Gly/N-degron识别与底物蛋白泛素化降解研究；2. 阐明ZYG11A通过调控细胞周期关键蛋白影响胰腺癌细胞增殖和迁移侵袭的机制；3. ZYG11A在有丝分裂滑移中的功能及其对抗有丝分裂化疗药物疗效的影响；4. 探讨ZYG11A在IGF1/p53信号轴下游的作用，为胰腺癌靶向治疗提供新策略；5. 研究ZYG11A基因突变对蛋白功能的影响及疾病关联

参考文献

1. Wang X et al. ZYG11A serves as an oncogene in non-small cell lung cancer and influences CCNE1 expression. Oncotarget, 2016. PMID:26771237

2. Achlaug L et al. ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease. Frontiers in Endocrinology, 2021. PMID:34220714

3. Achlaug L et al. Identification of ZYG11A as a candidate IGF1-dependent proto-oncogene in endometrial cancer. Oncotarget, 2019. PMID:31320996

4. Husni RE et al. DNA hypomethylation-related overexpression of SFN, GORASP2 and ZYG11A is a novel prognostic biomarker for early stage lung adenocarcinoma. Oncotarget, 2019. PMID:30899432

5. Balachandran RS et al. The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage. J Cell Biol, 2016. PMID:27810909

6. Charoensuk C et al. Autosomal dominant diabetes associated with a novel ZYG11A mutation resulting in cell cycle arrest in beta-cells. Mol Cell Endocrinol, 2021. PMID:33321115

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